GNAI1 antibody

Volume: 100 µg Purification: Immunogen affinity purified Form: liquid Purity: 95% as determined by SDS-PAGE Host: Rabbit Clonality: polyclonal Clone ID: Isotype: IgG Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.) Background: Guanine nucleotide-binding proteins(G proteins) function as transducers downstream of G protein-coupled receptors(GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins(PubMed: 8774883, PubMed: 18434541). Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels(By similarity). The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. Required for normal cytokinesis during mitosis(PubMed: 17635935). Immunogen: guanine nucleotide binding protein(G protein), alpha inhibiting activity polypeptide 1 Synonyms: Observed MW: 40 kDa Uniprot ID: P63096 Reactivity: Human, Mouse, Rat Tested Application: ELISA, WB, IHC, FC, IF Recommended dilution: WB: 1: 500-1: 2000; IHC: 1: 20-1: 200; IF: 1: 20-1: 200 Gene ID: 2770 Research Area: Signal Transduction