Cleaved-Caspase 3 (Asp175), p17 Antibody

Volume: 100 µL Host: Rabbit Clonality: Polyclonal Isotype: IgG GeneName: CASP3 GeneID: 836 Uniprot: P42574 Specificity: Cleaved-Caspase 3 (Asp175,p17) Antibody detects endogenous levels of fragment of activated Caspase 3 resulting from cleavage adjacent to Asp175. Immunogen: The antiserum was produced against synthesized peptide derived from human Caspase 3. Storage: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 13 months from date of receipt. Reactivity: Human,Mouse,Rat,Bovine Prediction: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Xenopus Application: WB,IHC,IF/ICC,ELISA(peptide) Dilution: WB 1: 500-1: 2000, IHC 1: 50-1: 200, IF/ICC 1: 100-1: 500, ELISA(peptide) 1: 20000-1: 40000 Conjugate: Unconjugated RRID: AB_2835326 An Introduction to Blocking Peptides: A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site. Cat# Blocking Peptide: AF7022-BP Des# Blocking Peptide: Cleaved-Caspase 3 (Asp175), p17 Blocking peptide