Mouse Apolipoprotein B100 (APO-B100) ELISA Kit

Reactivity: Mouse (Mus musculus) UniProt: N/A Abbreviation: APO-B100 Aliases: N/A Application: ELISA Range: 31.25-2000 ng/mL Sensitivity: 14.5 ng/mL Intra-AssayCV: ?3.7% Inter-AssayCV: ?7.0% Recovery: 1.07 Sample Type: Serum, Plasma, Other biological fluids Detection Method: Sandwich Analysis Method: Quantitative Test principle: This assay employs a two-site sandwich ELISA to quantitate APO-B100 in samples. An antibody specific for APO-B100 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAPO-B100 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for APO-B100 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of APO-B100 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview: Stability: